Holderness has a long, successful tradition of competitive lacrosse throughout New England. The foundation of this success has been the JV program. The team competes in the Lakes Region League of the New England Prep School Athletic Council and plays from the end of March until the end of May.

At the junior varsity level, emphasis is placed on skill development as well as teamwork and sportsmanship. Daily practices include stick skills, shooting, and conditioning drills as well as defense and system work. The Holderness JV lacrosse program provides players with the opportunity to grow and mature and test their skills against top-level New England competition. It is both a stepping stone for those with aspirations to play at the varsity level as well as a competitive program for those only playing for the love of the game.